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CRISPR-Cas: A Laboratory Manual. Jennifer Doudna

CRISPR-Cas-A-Laboratory-Manual.pdf
ISBN: 9781621821311 | 450 pages | 12 Mb

• CRISPR-Cas: A Laboratory Manual
• Jennifer Doudna
• Page: 450
• Format: pdf, ePub, fb2, mobi
• ISBN: 9781621821311
• Publisher: Cold Spring Harbor Laboratory Press

Download CRISPR-Cas: A Laboratory Manual

Books and magazines download CRISPR-Cas: A Laboratory Manual

CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

Addgene: CRISPR/Cas9 Guide
Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. The CRISPR/Cas Immune System Is an Operon Regulated by LeuO
The results obtained show that in S. Typhi the CRISPR/Cas system is a LeuO- dependent operon silenced Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Lab Press Cell Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Mouse Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. Cold Spring Harbor Lab Press Developmental Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego. CRISPR-Cas: A Laboratory Manual - Cold Spring Harbor Laboratory
Featuring contributions from leading figures in the CRISPR–Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes   Expression of CRISPR/Cas single guide RNAs using - Cshlp.org
The in vivo application of CRISPR/Cas-based DNA editing technology will require Molecular Cloning: A Laboratory Manual (Fourth Edition) 

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